Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Quercetin inhibits porcine intestinal inflammation in vitro

Zhi-Gang Chen¹, Guang-Ren Xu¹, Qiao-Ling Yuan¹, Hui-Yu Chen², Hong-Yu Lei², Jian-Ming Su¹

¹Department of Basic Veterinary Medicine; ²Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 410128, PR China.

For correspondence:- Jian-Ming Su Email: sjmauhn@163.com

Accepted: 24 September 2018 Published: 31 October 2018

Citation: Chen Z, Xu G, Yuan Q, Chen H, Lei H, Su J. Quercetin inhibits porcine intestinal inflammation in vitro. Trop J Pharm Res 2018; 17(10):1947-1952 doi: 10.4314/tjpr.v17i10.8

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effect of Quercetin (Que) on inflammatory reaction in intestinal porcine enterocyte cells, IPEC-J2, induced by lipopolysaccharide (LPS).

Methods: IPEC-J2 cells were pretreated with Que and then incubated with LPS. Cell viability, cell morphology, nitric oxide (NO) content, gene expression levels of interleukin-6 (IL-6) and interleukin-8 (IL-8), were then evaluated.

Results: LPS incubation at 10 μg/mL for 24 h showed no effect on the viability and cell morphology of IPEC-J2, while Que pre-incubation significantly enhanced the viability of IPEC-J2 and improved cell morphology (p < 0.05). Que decreased the expression levels of pro-inflammatory cytokines IL-6 mRNA and IL-8 mRNA raised by LPS (p < 0.05). Interestingly, both LPS and Que demonstrated no influence on the release of NO.

Conclusion: Pre-treatment of Que shows a positive effect on intestinal porcine enterocyte cells and inhibited porcine intestinal inflammation in vitro induced by LPS, but the mechanism may be not associated with NO-related signaling pathway. Therefore, Que might have a potential effect as a veterinary drug or feed additive for the treatment of enteritis in pigs.

Keywords: Quercetin, Inflammatory cytokines, Intestinal porcine enterocyte cells, Lipopolysaccharide